Impact of deletions in the TNF-α 3'UTR on IFIT-2 mediated inhibition of protein expression. RAW and RAW-IFIT-2 cells were co-transfected with expression vectors encoding GFP with no 3'UTR (GFP) or GFP with the indicated 3'UTRs and an expression vector encoding cherry with no 3'UTR for four hours. (A) depicts the constructs used, (B) shows the mean of several experiments (n = 3–8) of the percentage of reduction of the mean GFP fluorescence of RAW-IFIT-2 compared to RAW264.7 cells for each construct used. Asterisks indicate significant differences (p < 0.05, one-way ANOVA, Dunnett test) compared to control (GFP, no UTR). (C) illustrates the histograms of the GFP fluorescence of one representative experiment.
Berchtold et al. BMC Immunology 2008 9:75 doi:10.1186/1471-2172-9-75