Impact of 3'UTRs on IFIT-2 mediated inhibition of protein expression. RAW and RAW-IFIT-2 cells were co-transfected with expression vectors encoding GFP with no 3 'UTR (GFP) or GFP with the indicated 3'UTRs (TNF, IL-6, MIP-2, VEGF, IFIT-1) and an expression vector encoding cherry with no 3'UTR for four hours. Flow cytometry of cells was performed and gated on cherry expressing cells representing efficiently transfected cells. (A) shows the mean of several experiments (n = 3–8) of the percentage of reduction of the mean GFP fluorescence of RAW-IFIT-2 compared to RAW264.7 cells for each construct used. (B) illustrates the histograms of the GFP fluorescence of one representative experiment. Asterisks indicate significant differences (p < 0.05, one-way ANOVA, Dunnett test) compared to control (GFP, no UTR).
Berchtold et al. BMC Immunology 2008 9:75 doi:10.1186/1471-2172-9-75