Table 3

Antiendomysial (EmA) and transglutaminase-2 antibody (TG2-ab) secretion to organ culture supernatants after in vitro PT-gliadin challenge. Organ culture of treated celiac disease patient biopsy samples (n = 9) who had no small-bowel mucosal IgA deposits. Small-intestinal biopsy samples were cultured either with medium only or with PT-gliadin for 24 or 48 hours. EmA titers were graded according to intensity of staining as follows: negative (neg), weak positive (+) and strong positive (++, +++ or ++++).

EmA

TG2-ab (U)

Patient

GFD years

Medium 24 h

PT-gliadin 24 h

PT-gliadin 48 h

Medium 24 h

PT-gliadin 24 h

PT-gliadin 48 h

12

1

neg

neg

neg

2.0

4.8

13.3

13

2

neg

neg

neg

8.8

6.9

4.4

14

4

neg

neg

neg

2.2

6.2

10.3

15

4

neg

neg

neg

2.5

1.7

2.6

16

6

neg

neg

neg

8.1

7.7

12.4

17

7

neg

neg

neg

2.4

3.7

1.9

18

8

neg

neg

neg

2.5

1.3

1.7

19

14

neg

neg

neg

0.9

2.9

0.7

20

20

neg

neg

neg

11.6

2.5

11.6

Median

neg

neg

neg

Median

2.5

3.7

4.4*

GFD = gluten-free diet

* P > 0.05 compared to medium only 24 hours and PT-gliadin 24 hours

Stenman et al. BMC Immunology 2008 9:6   doi:10.1186/1471-2172-9-6