Table 2

Antiendomysial (EmA) and transglutaminase-2 antibody (TG2-ab) secretion to organ culture supernatants after in vitro PT-gliadin challenge. Organ culture of treated celiac disease patient biopsy samples (n = 11) who had small-bowel mucosal IgA deposits. Small-intestinal biopsy samples were cultured either with medium only or with PT-gliadin for 24 or 48 hours. EmA titers were graded according to intensity of staining as follows: negative (neg), weak positive (+) and strong positive (++, +++ or ++++).

EmA

TG2-ab (U)

Patient

GFD years

Medium 24 h

PT-gliadin24 h

PT-gliadin 48 h

Medium 24 h

PT-gliadin 24 h

PT-gliadin 48 h

1

1

++

+++

++++

11.0

17.1

37.8

2

1

++

++

+++

7.3

11.8

30.3

3

1

++

++

+++

16.0

6.3

26.6

4

1

+

++

+++

12.4

13.4

9.5

5

1

++

neg

++

17.2

7.4

22.9

6

1

neg

++

++

6.5

13.1

19.9

7

1

neg

neg

+

7.6

5.9

24.5

8

2

neg

+

++

7.8

5.3

14.8

9

3

+

++

++++

21.0

10.3

30.9

10

3

neg

++

++

4.9

7.0

nd

11

4

++

++

nd

24.5

11.9

nd

Median

+

++

+++*

Median

11.0

10.3

24.5†

nd = not determined

GFD = gluten-free diet

* P < 0.01; † P = 0.011 compared to medium only 24 hours and PT-gliadin 24 hours.

Stenman et al. BMC Immunology 2008 9:6   doi:10.1186/1471-2172-9-6