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Burst activities of differentiated cells expanded in IL-stimulated endothelial supernatant. |
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| Control |
E. coli |
fMLP |
PMA |
|
|
|
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| IL-1β (FBS) |
52.5 ± 13.1 (593 ± 156.9) |
509.5 ± 107.9 (169.1 ± 30.6) |
62.7 ± 10.5 (1336 ± 320.5) |
350.4 ± 95.5 (2873.4 ± 615.9) |
| IL-3 |
55.4 ± 13.2 |
800.7 ± 343.1 |
74 ± 33.1 |
784.3 ± 334.7 |
| IL-6 |
39 ± 9.4 |
467.6 ± 167.31 |
177 ± 124 |
287.1 ± 104.5 |
|
After culturing the cells for one week with G-CSF (100 ng/ml) and keeping them overnight in human serum, expanded cells showed a ten-fold increased burst activity in response to E. coli and PMA. Oxygen radical formation in cells from different interleukin conditions were comparable (p > 0.05). Shown are average results of mean fluorescence activities ± SE from five independent experiments. In brackets: mean results after storage in FBS-based medium (n = 10). PMA: phorbol 12-myristate 13-acetate; E. coli: Escherichia coli; fMLP: N-formyl-methionyl-leucyl-phenylalanin. | ||||
Moldenhauer et al. BMC Immunology 2008 9:56 doi:10.1186/1471-2172-9-56 |
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