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Open Access Highly Accessed Research article

Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice

Limei Li146, Zhaoliang Fei2, Jianke Ren12, Ruilin Sun12, Zhihui Liu1, Zhejin Sheng3, Long Wang2, Xia Sun2, Jun Yu5, Zhugang Wang2 and Jian Fei23*

Author Affiliations

1 Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, PR China

2 Shanghai Research Center for Model Organisms, Pu Dong, Shanghai, PR China

3 School of Life Science and Technology, Tongji University, Shanghai, PR China

4 Graduate School of Chinese Academy of Sciences, PR China

5 Shanghai Genomics, Inc, Shanghai, PR China

6 Shanghai Nan Fang Model Organism Research Center, No. 3577. Jinke Road, Pudong, Shanghai, People's Republic of PR China

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BMC Immunology 2008, 9:49  doi:10.1186/1471-2172-9-49

Published: 19 August 2008

Abstract

Background

Interleukin 1 beta (IL-1β) plays an important role in a number of chronic and acute inflammatory diseases. To understand the role of IL-1β in disease processes and develop an in vivo screening system for anti-inflammatory drugs, a transgenic mouse line was generated which incorporated the transgene firefly luciferase gene driven by a 4.5-kb fragment of the human IL-1β gene promoter. Luciferase gene expression was monitored in live mice under anesthesia using bioluminescence imaging in a number of inflammatory disease models.

Results

In a LPS-induced sepsis model, dramatic increase in luciferase activity was observed in the mice. This transgene induction was time dependent and correlated with an increase of endogenous IL-1β mRNA and pro-IL-1β protein levels in the mice. In a zymosan-induced arthritis model and an oxazolone-induced skin hypersensitivity reaction model, luciferase expression was locally induced in the zymosan injected knee joint and in the ear with oxazolone application, respectively. Dexamethasone suppressed the expression of luciferase gene both in the acute sepsis model and in the acute arthritis model.

Conclusion

Our data suggest that the transgenic mice model could be used to study transcriptional regulation of the IL-1β gene expression in the inflammatory process and evaluation the effect of anti-inflammatory drug in vivo.