Figure 3.

Amplification of differentiation signature genes in primary human T cells. (A) Expression level (aribrary units) of memory-phenotype marker genes (upper panel) or naive marker genes (lower panel) measured in sorted populations of naive (white bars) or memory-phenotype (black bars) peripheral blood CD4 T cells (25,000 cells/well). Each bar represents mean of ~6 replicate wells. Standard deviation was < 8% (not shown) in all cases. (B) Difference in expression level for naive (white bars) or memory-phenotype (black bars) marker genes between memory-phenotype and naive CD4 T cells. Values > 0 show relative increase in expression in memory-phenotype CD4 T cells; < 0 show relative increase in naive CD4 T cells. (C) Cumulative score of memory-phenotype (plotted on Y-axis) or naive marker genes (X-axis) in effector/memory phenotype (black symbols) or naive (white symbols) CD4 T cells. Values expressed as Z scores to compensate for wide variation in expression level of individual genes.