Comparison of human B cell activation by TLR7 and TLR9 agonists

doi:10.1186/1471-2172-9-39

BMC Immunology

Volume 9

Viewing options:

Abstract
Full text
PDF (382KB)
Additional files

Associated material:

Related literature:

Articles citing this article
on Google Scholar
on ISI Web of Science
on PubMed Central
Other articles by authors
on Google Scholar

Hanten JA
Vasilakos JP
Riter CL
Neys L
Lipson KE
Alkan SS
Birmachu W

on PubMed

Hanten JA
Vasilakos JP
Riter CL
Neys L
Lipson KE
Alkan SS
Birmachu W
Related articles/pages
on Google

on Google Scholar

on PubMed

Tools:

Post to:

Research article

Comparison of human B cell activation by TLR7 and TLR9 agonists

John A Hanten¹^,2 , John P Vasilakos¹^,3 , Christie L Riter¹ , Lori Neys¹^,4 , Kenneth E Lipson¹^,5 , Sefik S Alkan¹^,6 and Woubalem Birmachu¹^,7

¹Department of Pharmacology, 3M Pharmaceuticals, St. Paul, MN 55144, USA

²3M Drug Delivery Systems, St. Paul, MN 55144, USA

³Biothera, 3388 Mike Collins Dr, Eagan, MN 55121, USA

⁴DiaSorin, 1951 Northwestern Ave, P.O. Box 285, Stillwater MN 55082, USA

⁵FibroGen, Inc., 225 Gateway Blvd., South San Francisco, CA 94080, USA

⁶Alba Therapeutics Corp, 800 W. Baltimore St., Suite 400, Baltimore, MD 21201, USA

⁷3M Medical, St. Paul, MN 55144, USA

author email corresponding author email

BMC Immunology 2008, 9:39doi:10.1186/1471-2172-9-39


Published:	24 July 2008

Additional files

Additional file 1:

Flow cytometric analysis of human B cells. Flow cytometric analysis of human B cells. Panel A: B cells were enriched from human PBMC using immunomagnetic beads, and analyzed for purity by flow cytometry using CD-19 and BDCA-4 as markers (pre-sort purity was 80.3%, one representative donor). Panel B: The enriched B cell population was then further purified by flow sorting, and analyzed as described above (post-sort purity was 99.9%, one representative donor).

Format: PPT Size: 73KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Additional file 2:

Gene expression profile of human B cells modulated by TLR7, 8, or 9 agonists. Gene expression profile of human B cells from one donor (donor 1) after treatment with TLR7, 8, or 9 agonists. Gene expression was determined at 2, 8 and 24 hours post stimulation.

Format: DOC Size: 130KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 3:

Interferon and interferon-inducible genes in human B cells treated with TLR7, 8, 9 agonists. Gene expression profile of human B cells from one donor (donor 1) after treatment with TLR7, 8, or 9 agonists. Interferon and interferon inducible genes were minimally and inconsistently modulated by treatment with TLR agonists. Gene expression was determined at 2, 8 and 24 hours post stimulation.

Format: DOC Size: 49KB Download file

This file can be viewed with: Microsoft Word Viewer