Table 1

Counts of macrophages and dendritic cells of the small intestine.

Experimental groups
Macrophages
Dendritic cells

Nursing





12 days
Group A

45 ± 12b
37 ± 12b

Group B

24 ± 7a,c
16 ± 4a
21 days
Group A

22 ± 6a,c,d
12 ± 7a,c

Group B

25 ± 8a,c
12 ± 3a,c

Weaning

28 days
Group A
Aa
22 ± 7a,c,d
9 ± 2c


Ab
14 ± 3d
11 ± 2a,c

Group B
Ba
23 ± 7a,c,d
20 ± 7a


Bb
18 ± 4c,d
16 ± 6a,c
45 days
Group A
Aa
32 ± 6a
14 ± 4a


Ab
72 ± 10e
24 ± 3b

Group B
Ba
45 ± 13b
18 ± 2a


Bb
56 ± 10b,e
27 ± 6b

Macrophages and dendritic cells were determined by indirect immunofluorescence on the small intestine tissue slides of mice from different experimental group (Mother during suckling period without PFM (A) or with PFM (B) and offspring after weaning without PFM (a) or with PFM (b). Macrophages were determined using the BM8 monoclonal antibody which reacts with mouse F4/80 antigen and dendritic cells were determined using the 33D1 monoclonal antibody which recognizes a mouse dendritic cell-specific surface marker.

Results are expressed as number of positive cells recognized for the respective primary antibody, counted in 10 fields of vision at 1000× of magnification. Values are means for n = 15 ± SD mice from each group and at each time point. a,b,c,d,e Means for each cell population without a common letter differ significantly (P < 0.05).

de Moreno de LeBlanc et al. BMC Immunology 2008 9:27   doi:10.1186/1471-2172-9-27