Table 1 |
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|
Direct and his-tag ELISAs for IGFBP-2 antibodies meet validation standards. |
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|
Parameter evaluated |
Assay for antibodies to IGFBP2 |
Standard |
|
|
|
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|
Indirect |
His-tag |
||
|
|
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|
Limit of detection, ug/ml |
0.0005 |
0.0005 |
N/A |
|
Intra-assay cv |
12% |
13% |
<15% |
|
Inter-assay cv |
11% |
14% |
<15% |
|
Accuracy |
93% |
88% |
|
|
(technical specificity) |
(91%) |
(83%) |
>90% |
|
(technical sensitivity) |
(100%) |
(100%) |
|
|
Calibration curve |
r2 = 0.99 |
r2 = 0.99 |
r2 = 0.95 |
|
cv = 7% |
cv = 8% |
cv <15% |
|
|
Reference range, ug/ml |
0.025+/-0.148 |
0.024+/-0.051 |
N/A |
|
|
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|
Goodell et al. BMC Immunology 2008 9:23 doi:10.1186/1471-2172-9-23 |
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