Dendritic Cells are preferentially targeted among hematolymphocytes by Modified Vaccinia Virus Ankara and play a key role in the induction of virus-specific T cell responses in vivo

Luzheng Liu¹^,2 , Rahul Chavan²^,3 and Mark B Feinberg²^,4

¹Harvard Skin Disease Research Center, Department of Dermatology, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, USA

²Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30329, USA

³University of South Florida College of Medicine, Tampa, FL 33612, USA

⁴Merck Vaccine Division, West Point, PA 19486, USA

author email corresponding author email

BMC Immunology 2008, 9:15doi:10.1186/1471-2172-9-15


Published:	15 April 2008

Additional files

Additional file 1:

IFN-α was not detected in the supernatant of VV-infected DCs. (A) Immature BMDCs or purified CD11c⁺splenic DCs were either mock treated or infected with rVV-EGFP (a kind gift of Dr. Lawrence Corey, University of Washington, Seattle, WA) at a MOI of 10. Supernatant samples were collected at 10 and 24 h post infection, and IFN-α levels were measured by ELISA. These data are representative of three independent experiments. (B) VV-infected BMDCs produced a soluble factor(s) that abrogated the detection of IFN-α in the supernatant. Immature BMDCs were infected with rMVA-GFP or rVV-EGFP at a MOI of 10. Supernatant samples were collected at 24 h post infection. Various amounts of rmIFN-α were added into supernatant samples and ELISA was subsequently performed to detect IFN-α.

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