  Research articleSexual dimorphism in immune response genes as a function of pubertyRebecca Lamason1 1 Division of Rheumatology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA 2 Research Center for Genetic Medicine, Children's National Medical Center, 111 Michigan Ave, NW, Washington DC, 20010, USA 3 National Institutes of Health, Bethesda, MD, USA 4 University of Pennsylvania, Philadelphia, PA, USA
BMC Immunology 2006, 7:2doi:10.1186/1471-2172-7-2
Additional filesAdditional File 1: Genes up regulated during puberty in male and female mice. Format: DOC Size: 26KB Download file This file can be viewed with: Microsoft Word Viewer Additional File 2: Genes down regulated during puberty in male and female mice. Format: DOC Size: 38KB Download file This file can be viewed with: Microsoft Word Viewer Additional File 3: Genes differentially expressed in post-pubertal male and female mice. Format: DOC Size: 40KB Download file This file can be viewed with: Microsoft Word Viewer Additional File 4: Effect of estrogen on FasL expression in activated CD8+ T cells. Purified CD8+ T cells were isolated and stimulated with plate-bound CD3/CD28 in the presence and absence of estrogen (10-8M) for 24 h. The cells were stained with PE-labeled anti-FasL antibodies. Filled area, isotype control; green line, CD3/CD28-stimulated cells; pink line, CD3/CD28 and estrogen. Format: TIFF Size: 16KB Download file Additional File 5: IFN-γ levels in activated CD8+ T cells. Purified CD8+ T cells were cultured in the presence and absence of plate-bound Fas-Fc/CD3/CD28 in the presence and absence of estrogen. IFN-γ was estimated using a commercial ELISA kit. Format: JPEG Size: 69KB Download file |
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