Table 3

Intergenic difference of RT efficiencies is not due to interassay variation. Serial 1:10 dilutions of a single cellular RNA sample, of a pooled RNA sample and of a pooled DNA sample were used to generate standard curves for different targets (TNF-α, β2-MG, MIF and UBC). Slopes were indicative of amplification efficiencies, which were normalised to 100% for the pooled DNA sample for easier comparison to amplification efficiencies of the two other samples.

Single cellular RNA sample

Pooled RNA sample

Pooled DNA sample


TNF-α

95

95

100

β2-MG

90

90

100

MIF

88

88

100

UBC

81

80

100


Boeuf et al. BMC Immunology 2005 6:5   doi:10.1186/1471-2172-6-5

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