Figure 3.

AP^pos cells express the FDC-marker CD35 and the myeloid marker CD68. Monocytes were cultured under conditions described in Fig. 1 for differentiating into FDC-like cells (a) or for macrophages (b) and triple-stained for AP, CD 35 and CD68. a, FDC-like cells co-expressing CD68 (red; filled arrow), AP (blue; open arrow), and CD35 (brown; arrowhead) at different degrees; b, control cells cultured in parallel in the presence of macrophage differentiation conditions stained for AP (negative), CD35 (negative), CD68 (positive, red).