Correlation of qPCR and Flow Cytometry. Correlation of qPCR and flow cytometric analysis of for detection of OT-1 cells after dilution into wild-type C57BL/6 spleenocytes. The cell dilutions were calculated to represent 4%, 1,3%, 0.433%, 0.144%. 0.048%, and 0.016% total OT-1 CD8+T cells.
Wright et al. BMC Immunology 2005 6:20 doi:10.1186/1471-2172-6-20