Open Access Research article

Expression of human AID in yeast induces mutations in context similar to the context of somatic hypermutation at G-C pairs in immunoglobulin genes

Vladimir I Mayorov1, Igor B Rogozin23, Linda R Adkison1, Christin Frahm5, Thomas A Kunkel4 and Youri I Pavlov567*

Author Affiliations

1 Mercer University School of Medicine, Macon, GA 31207, USA

2 National Center for Biotechnology Information NLM, National Institutes of Health, Bethesda MD 20894, USA

3 Institute of Cytology and Genetics SD RAS, Novosibirsk 630090, Russia

4 Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

5 Eppley Institute for Research in Cancer, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198, USA

6 Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198, USA

7 Department of Pathology and Microbiology, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198, USA

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BMC Immunology 2005, 6:10  doi:10.1186/1471-2172-6-10

Published: 10 June 2005

Additional files

Additional File 1:

Spectra of can1 mutations in yeast. Types of nucleotides changes found are shown above the DNA sequence of the non-transcribed strand. A. ung1 strain, B. wild-type strain. Most of the mutations were single base pair substitutions. The multiple mutations found in the CAN1 are listed below. wild-type strain 452,C->T; 1728,T->G 123,A->G; 1392,G->A 123,A->G; 412,C->T 589,T->A; 1151,C->G 1485,G->T; 1486,C->A ung1 strain 553,C->A; 1633,C->T 1018,G->A; 1728,T->G 299,G->A; 1475,C->T 424,G->A; 980,G->A 572,T->C; 573,C->T 509,G->A; 516,G->A; 806,G->A

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