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Table 4
Primer sequences and detailed PCR conditions used to generate standard recombinant DNA.
Gene name		5'-3' primer sequence	Position^a(cDNA)	Annealing temperature^b

IL1a	FW	GTGGTGGTGTCAGCAACATCAAAC	275–862	56°C
	RW	GAAATCTATCATGGAGGGCAGTCC
IL1b	FW	TGAAAGCTCTCCACCTCAATGGAC	501–894	57°C
	RW	TGCAGCCATCTTTAGGAAGACACG
IL1RA	FW	AAGACCTTCTACCTGAGGAACAACC	139–310	55°C
	RW	GCTTGGTGTCATCTCCAGACTTG
IL1R1	FW	TGTCTACTGGAAGTGGAATGGGTC	1143–1500	56°C
	RW	GGGAAGAAAATCAGAGCAGGAGTC
IL1R2	FW	CACCCAGTTCTTGGAGACGATTG	226–598	57°C
	RW	TGGAGGAGAGAGCTGAGATTTGC
IL6	FW	TCTGGAGTTCCGTTTCTACCTGG	388–682	55°C
	RW	CATAGCACACTAGGTTTGCCGAG
IL6R	FW	AGCAGGCAATGCTACCATTCAC	264–873	57°C
	RW	GTCGGTATCGAAGCTCGAATTG
TNFa	FW	AGCACAGAAAGCATGATCCGAG	4–499	58°C
	RW	CCTGGTATGAAGTGGCAAATCG
TNFR2	FW	TCAGATGTGCTGTGCTAAGTGTCC	93–512	58°C
	RW	GCCAGGATGCTACAAATGCG
SOCS3	FW	ATGGTCACCCACAGCAAGTTTC	18–679	56°C
	RW	TACTGGTCCAGGAACTCCCGAATG

Abbreviations: see Tables 1, 2. These primer sets allow generating recombinant DNA to ensure the specificity of the PCR amplification or to generate standard curves. ^aPosition of amplification product within cDNA sequence. Genbank accession number are given in Tables 1, 2, ^bmelting temperature of specific PCR product.
Peinnequin et al. BMC Immunology 2004 5:3 doi:10.1186/1471-2172-5-3

Table 4