|
Effects of DC activation on priming of CD8+ T cell responses |
|||||
| Exp. (a) |
DC used in priming (b) |
Percent of CD8+ cells binding Flu-MP-HLA-A2 tetramer |
Absolute number of Flu-MP specific cells |
Fold expansion Flu-MP specific T cells |
|
|
|
|||||
| 1 |
CD40L + IL-1β |
2.39 |
5020 |
Not determined |
|
| CD40L |
1.68 |
2900 |
" |
||
| Immature |
0.84 |
1190 |
" |
||
|
|
|||||
| 2 |
CD40L + IL-1β |
0.28 |
1400 |
7.0 |
|
| CD40L |
0.24 |
528 |
2.6 |
||
| Immature |
0.25 |
656 |
3.3 |
||
|
|
|||||
| 3 |
CD40L + IL-1β |
0.26 |
594 |
2.9 |
|
| CD40L |
0.14 |
309 |
1.5 |
||
| Immature |
0.00 |
0 |
0 |
||
|
|
|||||
| 4 |
CD40L + IL-1β |
IgG |
1.39 |
2870 |
19.1 |
| CD40L |
" |
0.64 |
945 |
6.3 |
|
| Immature |
" |
0.61 |
1130 |
7.5 |
|
| CD40L + IL-1β |
Anti-IL-12 |
0.57 |
1270 |
8.5 |
|
| CD40L |
" |
0.78 |
1200 |
8.0 |
|
| Immature |
" |
0.41 |
700 |
4.7 |
|
|
(a) Neonatal umbilical cord blood was used for exp. 1–3, adult mobilized peripheral blood was used in exp. 4. (b) Monocyte-derived DC cultured in GM-CSF+IL-4 were used as immature DC (unstimulated), or after activation with CD40L (1 ug/ml) or CD40L + IL-1β (1 ug/ml, 10 ng/ml respectively) for 24 hours. In experiment 4, polyclonal neutralizing Abs to IL-12 or normal goat IgG (1 μg/ml), were added at time of priming. | |||||
Wesa and Galy BMC Immunology 2002 3:14 doi:10.1186/1471-2172-3-14 |
|||||