Figure 7.

CD40L+IL-1β stimulated DC induce high levels of IFN-γ in MLR. DC were stimulated as indicated, then washed prior to culture with purified, allogeneic T cells. T cell priming was assessed by measuring IFN-γ producing cells with flow cytometry after a brief restimulation on anti-CD3/anti-CD28 coated plates. Data represent results of three independent experiments.