Figure 6.

CD40L+IL-1β stimulated DC are highly effective APCs for CD8⁺ T cell priming. HLA-A2⁺ cord blood-derived immature DC were treated with CD40L+IL-1β, CD40L or medium for 16 hours, pulsed with Flu-MP peptide, washed and used to prime naïve autologous T cells. On day 7, T cell cultures were analyzed for the presence of antigen-specific CD8⁺ T cells using Flu-MP-HLA-A2 tetramers. Representative dot plots are shown of CD8 vs. Flu-MP tetramer (A), and CD45RA vs. Flu-MP tetramer (gated on CD8⁺ cells) (B). All were gated on live, PI^- cells; quadrants were set based on background staining with control mAbs. Percent positivity is indicated in corresponding quadrants.