The production of cytokine mRNA determined by quantitative by PCR is shown for (A) HIV+ donor CD8+ T cells and (B) aged donor CD8+ T cells. The cDNA copies per ug RNA from the CD8+ T cells. The cDNA copies per ug RNA from the CD8+ T cells were derived from: 1, nonativated controls; 2, activated controls (INF-γ); 3, activated HIV+ or aged (INF-γ); 4, activated controls (TNF-α); 5, HIV+ or aged (TNF-α).
Eylar et al. BMC Immunology 2001 2:10 doi:10.1186/1471-2172-2-10