Open Access Highly Accessed Research article

Limited role for ASC and NLRP3 during in vivo Salmonella Typhimurium infection

Hanna K De Jong12*, Gavin CKW Koh23, Miriam HP van Lieshout12, Joris JTH Roelofs4, Jaap T van Dissel5, Tom van der Poll126 and W Joost Wiersinga126

Author Affiliations

1 Department of Internal Medicine, Division of Infectious Diseases and Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Meibergdreef 9, Room G2-132, Amsterdam, 1105, AZ, the Netherlands

2 Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center, Meibergdreef 9, Room G2-132, Amsterdam, 1105, AZ, the Netherlands

3 Department of Infection and Tropical Medicine, Heartlands Hospital, Bordesley Green East, Birmingham B9 5SS, UK

4 Department of Pathology, Academic Medical Center, Meibergdreef 9, Room G2-132, Amsterdam, 1105, AZ, the Netherlands

5 Department of Infectious Diseases, Leiden University Medical Center, Leiden, 2300, RC, the Netherlands

6 Department of Medicine, Division of Infectious Diseases, Academic Medical Center, Meibergdreef 9, Room G2-132, Amsterdam, 1105, AZ, the Netherlands

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BMC Immunology 2014, 15:30  doi:10.1186/s12865-014-0030-7

Published: 13 August 2014

Abstract

Background

The inflammasome is an intracellular protein complex triggered by exposure to intracellular pathogens, its components or other endogenous proteins. It leads to the activation of and subsequent release of proinflammatory cytokines such as IL-1β and IL-18. S. Typhimurium is a Gram-negative intracellular bacterium, which is known to trigger inflammasome assembly via recognition by the cytosolic receptors, NLRP3 and NLRC4 (which act via the adaptor protein, ASC) to induce cell death and cytokine release. We sought to characterize the role of ASC and NLRP3 in two different murine models (typhoid and colitis) of systemic Salmonella infection.

Results

Release of the inflammasome cytokine IL-18 was hampered in Asc−/− but not Nlrp3−/− mice (background C57BL/6) during S. Typhimurium infection. Unexpectedly, neither ASC nor NLRP3 played a significant role in host defense against S. Typhimurium infection, as reflected by equal bacterial counts in WT, Asc−/− and Nlrp3−/− mice at all time points, in both the typhoid and colitis models. Proinflammatory cytokine levels (TNF-α, IL-6) and the extent of hepatic and splenic pathology did not differ between groups in the typhoid model. In the colitis model small differences were seen with regard to splenic and hepatic inflammation, although this was IL-18 independent.

Conclusions

IL-18 release was reduced in Asc−/− but not Nlrp3−/− mice during S. Typhimurium infection. Despite this reduction, bacterial counts, cytokine levels and histological inflammation did not differ between wild-type and knockout mice in either model. Our results reveal a limited role for ASC and NLRP3 during in vivo S. Typhimurium infection despite its role in cytokine maturation.

Keywords:
Inflammasomes; Salmonella Typhimurium; Host-pathogen interactions