Open Access Highly Accessed Research article

Characterization of murine macrophages from bone marrow, spleen and peritoneum

Changqi Wang1*, Xiao Yu12, Qi Cao1, Ya Wang1, Guoping Zheng1, Thian Kui Tan1, Hong Zhao13, Ye Zhao1, Yiping Wang1 and David CH Harris1

Author Affiliations

1 Centre for Transplant and Renal Research at Westmead, Sydney, NSW, Australia

2 Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China

3 Department of Biochemistry and Molecular Biology, Shanxi Medical University, Shanxi, PR China

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BMC Immunology 2013, 14:6  doi:10.1186/1471-2172-14-6

Published: 5 February 2013

Additional files

Additional file 1:

SPMs, BMs and PMs were generated respectively, and then stained with anti-F4/80 and anti-CD11c, the gating of F4/80+ and CD11c-cells was based their isotype controls. Data are representive of 5 separate experiments.

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