Additional file 2: Figure S1.

Kinetics of CD14 and TLR-4 surface expression on monocytes, macrophages and iDCs/DCs upon stimulation with LPS, proTα or proTα(100–109). Monocytes, macrophages and iDCs (0 h) were stimulated with LPS (A), proTα (B), or proTα(100–109) (C) for 15 min, 30 min, 1 h and 18 h and assessed for the surface expression of CD14 and TLR-4 using flow cytometry. MFI values in the presence of neutralizing anti-TLR-4 Ab (+ a-TLR-4) are shown below each histogram. Histograms are from one representative donor of 3 tested. Using the loss of cell surface expression as a readout for TLR-4 and CD14 endocytosis from 0–36 h [31], data from all three donors are shown as mean values ± SDs for TLR-4 (D, E, F) and CD14 (G, H, I).

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Ioannou et al. BMC Immunology 2013 14:43   doi:10.1186/1471-2172-14-43