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DNA vaccines targeting the encoded antigens to dendritic cells induce potent antitumor immunity in mice

Jun Cao1, Yiqi Jin2, Wei Li3, Bin Zhang4, Yang He1, Hongqiang Liu1, Ning Xia1, Huafeng Wei5* and Jian Yan1*

Author Affiliations

1 Interventional Oncology, Dahua Hospital, Xuhui District, Shanghai 200237, China

2 Interventional Radiology, Suzhou Municipal Hospital, Suzhou, Jiangsu Province 215002, China

3 Department of Geriatric Neurology, Brain Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China

4 Department of Institution of Hepatobililary and Gastrointestinal Diseases, Second Artillary General Hospital, Beijing 100088, China

5 International Joint Cancer Institute, Second Military Medical University, Shanghai 200433, China

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BMC Immunology 2013, 14:39  doi:10.1186/1471-2172-14-39

Published: 14 August 2013

Additional files

Additional file 1: Figure S1:

Construction and expression of pcDNA3.1-scFvNLDC-145-EGFP and pcDNA3.1-EGFP a generated pcDNA3.1-scFvNLDC-145-EGFP by replacing the HER2 fragment with EGFP sequence cloned from pEGFP-N1 plasmid. The pcDNA3.1 vector encoding EGFP without DC-targeting scFv fragment as control. b 293T cells grown in 24-well plate were transfected with the two expression vectors using Lipofectamine 2000 (invitrogen). Green fluorescent protein GFP was observed by inverted fluorescence microscope (X51-A21PH, OLYMPUS).

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Additional file 2: Figure S2:

Analysis of cell population responsible for the protective effects by targeted vaccine. Animals (5 mice per group) were vaccinated with scFvNLDC-145-HER2 on days -21 and -7. On day 0, mice were inoculated s.c. with D2F2/E2 tumor cells. For in vivo depletion of CD4+, CD8+ T cells or CD19+ B cells, an anti-CD4 (0.5 mg/mouse; Clone GK1.5), anti-CD8 (0.5 mg/mouse; Clone YTS 169.4), anti-CD19 (0.2 mg/mouse; Clone 1D3) or control (0.5 mg/mouse; Clone 2A3) mAb was injected i.p. on days -7, -3 and -1 and repeated weekly later. All mAbs were purchased from BioXcell. Tumor developments were monitored, and animal survival was calculated.

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