A histogram representing changes in ROS production in the neutrophils of peripheral blood of study patients after stimulation with different concentrations of PMA. Data are presented as mean fluorescence intensity (MFI) ± SEM. *P < 0.05, when compared with COPD and healthy individuals groups; §P < 0.05, when compared with healthy individuals. The representative dot plot of neurophil population isolated from peripheral blood of healthy individuals (A), patients with COPD (B), patients with lung cancer/COPD (C) and patients with lung cancer (D) stimulated upon 30 nM of Phorbol 12-myristate 13-acetate (PMA). Side light scater (SSC) represents the granularity, complexity of the cells; FSC – forward light scatter (FSC) represents cell size; dihydrorhodamine-123 (DHR-123) a green fluorescent compound showing H2O2 intensity in neutrophils.
Vaguliene et al. BMC Immunology 2013 14:36 doi:10.1186/1471-2172-14-36