IL-17 does not induce Annexin-V or PI uptake. NSCs cells were cultured in 6-well plates and treated with IL-17 (25 ng/ml) for 48 h, followed by incubation with Annexin-V dye and PI and FACS analysis. (a) Representative histograms demonstrate the distribution of Annexin-V positive and PI positive cells in treated/untreated NSCs. (b) Plot depicts the percentage of Annexin-V positive and PI positive cells in treated/untreated NSCs. Data represent mean ± SE in duplicate samples from three identical experiments. NS, not significant.
Li et al. BMC Immunology 2013 14:20 doi:10.1186/1471-2172-14-20