Figure 2.

IL-17 induces a decrease in both neurosphere volume and cell number. NSCs were cultured at 400 cells/ml in 96- or 12-well plates and treated with IL-17, TNF-α and IL-10 for 96 h, as described in Materials and Methods. (a) NSCs treated with different concentrations of IL-17; (b) Number of NSCs treated with different concentrations of IL-17; (c) NSCs treated with IL-17 (25 ng/ml), TNF-α (25 ng/ml) and IL-10 (50 ng/ml) alone; (d) Number of NSCs treated with IL-17 (25 ng/ml),TNF-α (25 ng/ml) and IL-10 (50 ng/ml) alone. Cell number and the number of living neurospheres were measured using an inverse microscope. Mean percentages were determined from five independent experiments. NS, not significant.

Li et al. BMC Immunology 2013 14:20   doi:10.1186/1471-2172-14-20
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