Antigenicity of rBlo t 5 and rBlo t 21. IgE antibody levels were assessed by means of indirect ELISA in sera of atopic children (A) and asthmatic adult individuals (B) with antibodies against whole B. tropicalis extract. Each symbol represents the result obtained with an individual serum. Short horizontal solid lines represent the median value of the group results. The cut-offs are represented by horizontal dotted (0.269 for BtE assay), dashed (0.250 for rBlo t 5 assay) or solid (0.136 for r Blo t 21) lines, respectively. A) ** rBlo t 5 compared with Bt extract, p < 0.0067 and B)* rBlo t 5 compared with Bt extract, p < 0.0314, ** rBlo t 5 plus rBlo t 21 compared with Bt extract, p < 0.0034, Wilcoxon signed rank test.
Carvalho et al. BMC Immunology 2013 14:11 doi:10.1186/1471-2172-14-11