Effect of ATG therapy on antigen-stimulated T cell proliferation and cytokine production. NOD mice were treated with ATG or isotype IgG along with intraperitoneal injections of KLH in Alum as described in Methods. All mice were sacrificed at day 22 post treatment. A, spleen cells were incubated with KLH, BSA or media only; B, spleen cells were stimulated with anti-CD3 antibodies. All cultures were pulsed by 3H-thymidine (1 uCi/well) at day 4 for additional 16 hours. T cell proliferation was measured by scintillation counting. Triplicate wells were set up for each incubation. Three animals were included in each group. C, The production of cytokines in the cultures with KLH shown in Figure 6A was measured by Luminex assay. This experiment was repeated in additional two separate experiments with similar results.
Xia et al. BMC Immunology 2012 13:70 doi:10.1186/1471-2172-13-70