Figure 2.

ATG therapy differentially depletes naive and memory T cells in peripheral blood and spleen. NOD mice were treated with ATG or isotype IgG twice with a 3-day interval. At day 3 post treatment, CD62L+CD4+ and CD44+CD62L-CD4+ T cells in peripheral blood and spleen were examined by flow cytometry and analyzed by gating CD4+ T cells as depicted in the upper panels of A and B, respectively. A shows the summary of the percentages of CD62L+CD4+ T and CD44+CD62L- CD4+ T cells in total CD4+ T cells in the peripheral blood, respectively (lower panels). B shows the summary of the percentages of above T cell subsets in total CD4+ T cells in spleen cells, respectively (lower panels). Three mice were included in each group. Three independent experiments were performed with similar results.