Ex-vivo IL-17A production from γδ+T cells following neutrophil depletion. BALB/c mice received an intranasal inoculum of 1x104 CFU of C. neoformans strain H99γ in 50 μl of sterile PBS. Mice were treated with isotype control antibody or with anti-1A8 antibody. The lungs were excised at day 7 post-inoculation and a single cell suspension generated using enzymatic digestion. The leukocytes were isolated and then further purified for γδ+ T cells. The γδ+ T cells were plated at 1 × 105 cells/well and incubated for 24 h at 37°C, 5% CO2 with media alone (white bars) or with C. neoformans cell wall extract (CWE) (gray bars). Cell supernatants were removed and examined for IL-17A by ELISA. Asterisks (*) indicate where significant differences (P < 0.05) were observed between γδ+ T cells from mice treated with isotype control antibody compared to those from mice treated with the 1A8 neutrophil depletion antibody. Data are cumulative of three separate experiments.
Wozniak et al. BMC Immunology 2012 13:65 doi:10.1186/1471-2172-13-65