Table 6

NO2-production of J774 cells: Mouse macrophages untreated, treated with RJI-C, TB-KK or the MIX, stimulated with LPS, stimulated with LPS and treated with RJI-C, TB-KK or the MIX
Treatment Time of incubation (h)
24 48 72
No treatment 0.25 ± 0.04 0.69 ± 0.02 0.92 ± 0.2
RJI-C (15 μg/ml) (12 μM) 0.42 ± 0.03 0.75 ± 0.01 1.02 ± 0.3
TB-KK (15 μg/ml) (6.5 μM) 0.82 ± 0.05 1.25 ± 0.2 1.34 ± 0.2
MIX (RJI-C 9 μg/ml + TB-KK 6 μg/ml) (RJI-C 7.3 μM + TB-KK 2.6 μM) 0.72 ± 0.3 0.85 ± 0.3 1.06 ± 0.2
LPS (10 μg/ml) 2.93 ± 0.2 10.96 ± 0.4 12.16 ± 0.5
LPS + RJI-C (15 μg/ml) (12 μM) 2.85 ± 0.3 8.42 ± 0.1 10.21 ± 0.2
LPS + TB-KK (15 μg/ml) (6.5 μM) 3.12 ± 0.6 9.75 ± 0.1 11.45 ± 0.2
LPS + MIX (RJI-C 9 μg/ml + TB-KK 6 μg/ml) (RJI-C 7.3 μM + TB-KK 2.6 μM) 2.63 ± 0.4 7.25 ± 0.3 8.26 ± 0.1

Data are expressed as micromoles of NO2- for 106 input cells, and are means ± standard deviation of three different experiments each performed in triplicate.

Capparelli et al.

Capparelli et al. BMC Immunology 2012 13:61   doi:10.1186/1471-2172-13-61

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