Off-target mutation analysis. A Cel-I digest of heteroduplex DNA revealed no additional off-target mutations at the 10 loci with highest homology to Rag1; 1: Vom2r-ps62; 2: Atrn; 3: Vwa5b1; 4: KIAA2012; 5: Zfn9; 6: Dctn1; 7: Mylip; 8: Manl1; 9: LOC689553; 10: Kir3d1l; G/C: control heteroduplex DNA. B Genes, gene IDs, sequence homologies and localization of Rag1 related sequences to exclude off-target mutations; upper case: ZFN binding sites; lower case: ZFN cut site; homolog base pairs in red; (-): Gene located on the minus strand.
Zschemisch et al. BMC Immunology 2012 13:60 doi:10.1186/1471-2172-13-60