Association of iMo mobilization from bone marrow to periphery with IL-6 increases after CAWS administration. (A) Representative pathway showing the possible role of iMo releasing IL-6 after CAWS administration activating Th17 cells and inhibiting Treg cells in Ccr2+/+ mice. (B) Serum levels of IL-6 detected in Ccr2+/+ mice and Ccr2−/− mice, after full cycle of CAWS. (C) IL-6 levels in cell culture supernatants of splenocytes stimulated with anti-CD3/CD28 for 60 hours in Ccr2+/+ and Ccr2−/− mice, euthanized 10 days after the first cycle of CAWS administration. (D) FACS showing iMo (CD11b+, Ly6Chi, Ly6Glow) in bone marrow, (E) blood and (F) spleen in Ccr2+/+ and Ccr2−/− mice, 10 days after the first cycle of CAWS or PBS control.
Martinez et al. BMC Immunology 2012 13:56 doi:10.1186/1471-2172-13-56