Ccr2−/−mice, but not Ccr5−/− mice, are resistant to coronary/aortic inflammation after CAWS administration. (A-B) Representative diagram and histopathology of the five areas quantified for inflammation at the aortic root that included: right coronary artery (RCA), left coronary artery (LCA), right coronary cuspid (RCC), left coronary cuspid (LCC), and non-coronary cuspid (NCC). (C-D) Quantification of the inflamed area at the level of the root of the aorta. (E) Normal vascular structures showing the coronaries at the level of the root of the aorta in PBS-treated Ccr2+/+ (WT) mice. (F) Coronary and aortic inflammation in Ccr2+/+ mice after a full cycle of CAWS. (G) Decreased inflammation in Ccr2−/− mice, at the level of the root of the aorta, after CAWS administration (all pictures have a 4X magnification and were cropped for comparison purposes). (H) Incidence of coronary/aortic inflammation between Ccr2+/+, Ccr2−/−, and Ccr5−/− mice, after CAWS administration. (I) Number of areas affected in the coronary and aortic wall. (J) Total area inflamed (μm2) in each group of mice receiving two cycles of CAWS. Each bar represents the mean ± SE from a representative experiment out of 3 performed with 6–8 mice per group.
Martinez et al. BMC Immunology 2012 13:56 doi:10.1186/1471-2172-13-56