TCR rearrangement in developing T cell precursors in LmDL1-FL7 co-culture.A, TREC analysis in developing T cells. PCR analysis for TREC and Rag2 was performed using genomic DNA harvested at the indicated time points from co-cultures under different IL-7 conditions. B, Quantitative PCR analysis of TREC-positive cells in developing T cells. SYBR Green quantitative PCR analysis of TREC was performed using genomic DNA isolated from T cell precursors under indicated culture conditions. C, Analyses of Rag1, Rag2, TCF1 and LEF RNA expression. The expression of the indicated genes was detected by RT-PCR using 1ug of mRNA harvested from day 30 of the developing T cell precursors.
Patel et al. BMC Immunology 2012 13:46 doi:10.1186/1471-2172-13-46