Flow cytometry analyses of expression kinetics of CD8, CD4, CD7, and CD1a of the developing T cells under LmDL1 + IL-7 + Flt3L or LmDL1-FL7 co-culture condition. Schematic illustration of key stages of T cell development is shown at top. Adult human BM CD34+ HPCs were plated on LmDL1-FL7 or LmDL1 + IL-7 + Flt3L and surface expression of CD8, CD4, CD7 and CD1a was examined over time as depicted. The percentages of stained cells are indicated in the flow graph quadrons. ND, not done, due to insufficient amount of developing T cells from the early time points of the LmDL1 + IL-7 + Flt3L coculture.
Patel et al. BMC Immunology 2012 13:46 doi:10.1186/1471-2172-13-46