FFO suppresses causative factors in pruritus. (A) Mice were painted with 1% DNCB or vehicle on their abdomen as the first sensitization (day-7). On day 0, the mice were sensitized with 0.5% DNCB on their ears and were then sensitized with 0.5% DNCB every other day for up to 31 days. From day 12 until the completion of the experiment, mice were given FFO or NFO (100 mg/kg) in their drinking water, daily, and were painted with hydrocort on their ears every other day. The mice were sacrificed on day 32. (B) Mice were given 100 mg/kg FFO (•) or NFO (▴) daily and painted with hydrocort (−) every other day. The spontaneous scratching behaviors of the mice were recorded for 10 min with a digital camera on days 0, 10 and 31. (C and D) After sacrifice, the histamine and IgE in mouse serum was measured by ELISA. Data are representative of 8 mice per group. Values are mean ± S.D (n = 8 mice per group). **, *** compared to mice stimulated with DNCB alone (induction group). **P < 0.005; *** P < 0.001.
Han et al. BMC Immunology 2012 13:44 doi:10.1186/1471-2172-13-44