A. Cell proliferation following immunization with recombinantP. murinaMsg proteins MSG107Am and MSG119Am. Splenocytes from animals that were immunized with these recombinant Msg proteins were cultured in triplicate with each of the recombinant Msg proteins and (as a control) with recombinant protein encoded by the empty vector (without Msg). Groups of 4 animals received one, two or four injections with each antigen, as indicated by 1X, 2X, and 4X. Bars represent the stimulation index (SI) (geometric mean of results for Msg-stimulated response divided by empty vector-stimulated response). B. Antibody reactivity to the Msg variants measured by ELISA. Serum samples were run in duplicate for each antigen. Bars represent the mean optical density for 4 animals per immunization group. Unpaired t tests were used to compare the results for immunized animals to control (unimmunized or adjuvant alone) animals. Statistical significance is indicated by the following symbols: *, p ≤ 0.05; ‡, p ≤ 0.01; †, p ≤ 0.001.
Bishop et al. BMC Immunology 2012 13:39 doi:10.1186/1471-2172-13-39