Endocytic characteristics of DCs following treatment with ECM proteins. Endocytic ability of the DCs, at 48 h, was assessed by the uptake of FITC-dextran using flow cytometry (n = 2). Data were gated, based on forward/side scatter characteristics and on FITC channel to exclude dead cells and contaminating clumps of FITC-dextran. Error bars represent standard deviations. * p < 0.05. NB: Statistical analysis is based on differences between means of untreated control (CTRL) and treated samples. Sample treatments correspond to untreated (CTRL), 0.01% FN, 1% BSA and TG-2 activity defined as 0.039 U/mL (TG-2 [high]), 0.0036 U/mL (TG-2 [mid]) or 0.00022 U/mL (TG-2 [low]).
Dalleywater et al. BMC Immunology 2012 13:20 doi:10.1186/1471-2172-13-20