Analysis of DC phenotype following treatment with ECM proteins. (A) Flow cytometric analysis of the phenotype of untreated (control) DCs following culturing for 48 h (upper panel) and 72 h (lower panel). The filled histograms represent isotype or unstained controls, unfilled histograms show staining of named markers. The numbers are MFI values of a representative experiment (B) Phenotype of DCs untreated (CTRL) or treated with 0.01% FN, TG [high], TG [mid] or TG [low] for 48 h (upper panel) and 72 h (lower panel), (n = 3). MFI values for the indicated antigens are depicted as a percentage of the CTRL value. TG-2 activity defined as 0.039 U/mL (TG-2 [high]), 0.0036 U/mL (TG-2 [mid]) or 0.00022 U/mL (TG-2 [low]). Error bars represent standard deviations and * p < 0.05, ** p < 0.01, *** p < 0.001. NB: n = 2 for CD54 expression.
Dalleywater et al. BMC Immunology 2012 13:20 doi:10.1186/1471-2172-13-20