High glucose concentrations down-regulate CD33 expression in human monocytes. Monocytes from healthy donors were cultured in the presence of D-glucose (5.5, 15, 30, or 50 mmol/l) for 7 days. (A) Representative histograms show the expression of CD33 in monocytes cultured with 5.5 mmol/l (gray), 15, 30, or 50 mmol/l (open histogram) D-glucose. (B) Bar graphs show the MFI of CD33 in monocytes cultured under high glucose compared to those cultured in normal medium. The data are presented as the mean ± SD (n = 6). (C) Taqman gene expression mRNA analysis of CD33 and 18 S ribosomal RNA expression. The results were analyzed according to the ΔΔCt method, and all data are presented as the mean ± SD (n = 3). * P < 0.05 compared to 5.5 mmol/l D-glucose.
Gonzalez et al. BMC Immunology 2012 13:19 doi:10.1186/1471-2172-13-19