PMA/CD3 and PMA/CD28 stimulation differentially modulate primary T cell cytokine responses in human healthy donor blood. Healthy donor blood was stimulated with PMA/CD3 and PMA/CD28 and cultured for 24 hours in the presence or absence of pathway inhibitors for Lck and PKC. Culture supernatants were analyzed on A; IFNγ, IL-17 (Th1/17 cytokines) and B; IL-13 and IL-5 (Th2 cytokines). Figure C shows the involvement of Lck (420983; 1 μM) and PKC (AEB071; 1 μM) signal transduction on PMA/CD3-induced IFNγ production. Figure D shows the effect of Lck (A-420983; 1 μM)) and PKC (AEB071; 1 μM) signal transduction pathways on PMA/CD28-induced IL-13 production. Data are presented as results from 3 donors measured as biological duplicates. Significance of differences are presented as followed; * p < 0.05, **p < 0.01 using a one-way ANOVA with Dunnett's post hoc testing.
Smeets et al. BMC Immunology 2012 13:12 doi:10.1186/1471-2172-13-12