Heatmap of stimulation dependent gene clusters. Top left panel: CCL1 cluster characterized by induction of genes exclusively following CD28/PMA stimulation and subsequent repression by AEB071. Bottom left panel: IL-2 cluster characterized by induction of genes by all stimulations, and down-regulated by A420983 and AEB071 and to a lesser extent by CsA. Top right panel: EGR1 cluster characterized by induction of genes by all stimulations, but down-regulated only by AEB071 when stimulated with PMA/CD28 or PMA/CD3. A red color denotes an up-regulation, a green color a down-regulation. The MAPK inhibitors induced very little regulation and have been omitted from this figure for clarity. The gene lists shown in this figure, with extended annotation and their distance to the CCL1, IL-2 and EGR1 profiles are provided in Additional files: File S1, S2 and S3.
Smeets et al. BMC Immunology 2012 13:12 doi:10.1186/1471-2172-13-12