Flow cytometric analyses of stimulated CD4+ T cells. Purified CD4+ T cells were stimulated with PMA and ionomycin for 4-5 h in the presence of GolgiStop (monensin). The expression of CD300a/c was determined in the TCM (CD45RO+CD27+) and TEM (CD45RO+CD27-) cytokine producing cells. A representative healthy donor is shown for (A) the IFN-γ, IL-4 and IL-17a, and (B) IL-2 and TNF-α producing cells. Each graph represents the percentage of CD300a/c+ and CD300a/c- TCM and TEM cells that produce a given cytokine. Each symbol corresponds to a different donor.
Simhadri et al. BMC Immunology 2011 12:62 doi:10.1186/1471-2172-12-62