Figure 8.

Estimation of coxiella burden in recipient mice of antigen-pulsed BMDCs. Coxiella DNA copies were determined in five mouse samples per group by quantitative PCR. Data are expressed as the average copy number of five samples, and error bars indicate the standard deviation. Mice receiving phase I Ag-pulsed BMDCs (I Ag) and mock-pulsed BMDCs (Mock) were used as positive and negative controls, respectively. In quantitative PCR analysis, the copy number per mouse was obtained with 1% of the DNA sample extracted from 10 mg spleen tissue. Coxiella burden in mice receiving Com1-pulsed BMDCs was significantly lower than in mice receiving HspB-pulsed BMDCs (P<0.05). This result is from one independent experiment.