Measuring protein expression of Mafa-B*134:02 by fluorescent peptide. a) A fluorescent peptide, fGR9, was used to measure Mafa-B*134:02 expression in the PBMC. We looked at five different leukocyte subsets in 20 different animals. We determined relative expression using the following formula: (MFI fGR9)/(MFI No Peptide). b) MFI of W6/32 APC binding for each sample. c) Dot plots showing leukocytes where CD14 is plotted on the y-axis and peptide-FITC is plotted on the x-axis. Numbers to the right of the gates show the percentage of cells within the gate. Plots show standard error of the mean.
Greene et al. BMC Immunology 2011 12:39 doi:10.1186/1471-2172-12-39