Determination of apparent association constants for ROS-GAD65 by Langmuir plot. Langmuir plot of reciprocal of bound antigen concentration to antibody (1/r) versus reciprocal of free antigen concentration (1/[Ag]). Antigen and antibody binding between ROS-GAD65 and IgG isolated from nephropathic (-■-) serum no.3, retinopathic (-●-) serum no. 6 of complicated T1D and uncomplicated (-▲-) (serum no. 11) T1D subjects. Each value represents mean ± SD of four independent assays.
Khan et al. BMC Immunology 2011 12:19 doi:10.1186/1471-2172-12-19