Detection of serum autoantibodies against both native and modified GAD65 in all subjects. Direct binding ELISA of 1:100 diluted uncomplicated and complicated (nephropathy and retinopathy) T1D serum samples. Control samples were served as controls. Serum autoantibodies reactivity of all four groups was checked towards N-GAD65 (□) and ROS-GAD65 (■). Microtitre plates were individually coated with N-GAD65 and ROS-GAD65 (20 μg/ml). Each histogram represents the mean ± SD values.
Khan et al. BMC Immunology 2011 12:19 doi:10.1186/1471-2172-12-19